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The fluoresence signal from the tissue is small compare to the temporal
and spatial changes in biological systems, therefore, the researchers are
usually combine all information obtained during spectroscopic experiments
to find the unknown signatures. For example, the diffuse reflectance at the excitation light wavelength
can be analyzed together with a much weaker fluoresence signal. Below is shown a diagram for an instrumental setup to measure intensity of the DR spectra:
One, of course, needs to calibrate out the instrumental characteristics
measuring intensity of the lamp spectra in a similar instrumental setup:
Work was done while at Argose Inc. |
Can Diffuse Reflectance help?
